PRODUCT CODE: ER1510-11

Glut-1 Antibody (ER1510-11)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1,000 dilution.
  • Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1,000 dilution.
  • ICC staining Glut1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Glut1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Glut1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Glut-1 Antibody (ER1510-11)

Immunogen

Peptide.

Host

Rabbit

Positive Control

Human placenta tissue lysates, Hela, human liver tissue, human breast tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glut-1

SYNONYMS

Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody; CSE antibody; DYT17 antibody; DYT18 antibody; DYT9 antibody; EIG12 antibody; erythrocyte/brain antibody; Erythrocyte/hepatoma glucose transporter antibody; facilitated glucose transporter member 1 antibody; Glucose transporter 1 antibody; Glucose transporter type 1 antibody; Glucose transporter type 1, erythrocyte/brain antibody; GLUT antibody; GLUT-1 antibody; GLUT1 antibody; GLUT1DS antibody; GLUTB antibody; GT1 antibody; GTG1 antibody; Gtg3 antibody; GTR1_HUMAN antibody; HepG2 glucose transporter antibody; HTLVR antibody; Human T cell leukemia virus (I and II) receptor antibody; MGC141895 antibody; MGC141896 antibody; PED antibody; RATGTG1 antibody; Receptor for HTLV 1 and HTLV 2 antibody; SLC2A1 antibody; Solute carrier family 2 (facilitated glucose transporter), member 1 antibody; Solute carrier family 2 antibody; Solute carrier family 2, facilitated glucose transporter member 1 antibody

SEQUENCE SIMILARITIES

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

TISSUE SPECIFICITY

Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-226 by PKC promotes glucose uptake by increasing cell membrane localization.

SUBCELLULAR LOCATION

Cell membrane. Melanosome.

FUNCTION

Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.