PRODUCT CODE: ER1802-96

GLUR Antibody (ER1802-96)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Rat liver tissue
  • Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Rat liver tissue
  • ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with GLUR antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Rat liver tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GLUR Antibody (ER1802-96)

Immunogen

Recombinant protein within human glur 260-450 aa.

Host

Rabbit

Positive Control

A549, rat liver tissue, rat epididymis tissue, human liver tissue, human thyroid gland tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GLUR

SYNONYMS

GLR1 antibody; GLUR antibody; Glutathione reductase antibody; Glutathione reductase mitochondrial antibody; Glutathione reductase, mitochondrial antibody; GR antibody; Gr1 antibody; GRase antibody; GRD 1 antibody; GRD1 antibody; GSHR_HUMAN antibody; GSR antibody; MGC78522 antibody

SEQUENCE SIMILARITIES

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

SUBCELLULAR LOCATION

Cytoplasm. Mitochondrion.

FUNCTION

Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized Glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.