PRODUCT CODE: EM1701-34

Glucagon Mouse Monoclonal Antibody [5F1] (EM1701-34)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.
  • Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.
  • ICC staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Glucagon Mouse Monoclonal Antibody [5F1] (EM1701-34)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Mouse kidney tissue lysate, LOVO, PANC-1, SH-SY5Y, mouse pancreas tissue, rat pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5F1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa, additional band 55kDa

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glucagon

SYNONYMS

GCG antibody

SEQUENCE SIMILARITIES

Belongs to the glucagon family.

TISSUE SPECIFICITY

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

POST-TRANSLATIONAL MODIFICATION

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.