PRODUCT CODE: em140707

GFAP Monoclonal Antibody (EM140707)

  • IVD–IHC

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution.<br />
Positive control: Line 1: Rat brain Line2 :Human brain
  • Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution.<br />
Positive control: Line 1: Rat brain Line2 :Human brain
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.
  • ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution.
Positive control: Line 1: Rat brain Line2 :Human brain

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GFAP Monoclonal Antibody (EM140707)

Immunogen

Synthetic peptide within c-terminal of human gfap.

Host

Mouse

Positive Control

Mouse brain tissue, rat brain tissue, human brain tissue,A172 ,N2A ,Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-D4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC:1:200

  • IHC-P

  • 1:200

  • FC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Glial fibrillary acidic protein

GENE NAME

GFAP

SYNONYMS

GFAP

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in cells lacking fibronectin.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PKN1.

SUBCELLULAR LOCATION

Cytoplasm. Note=Associated with intermediate filaments.

FUNCTION

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.