PRODUCT CODE: ET1610-68

GART Recombinant Rabbit Monoclonal Antibody [SC66-02] (ET1610-68)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: 293T <br />
Lane 3: A431
  • Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: 293T <br />
Lane 3: A431
  • ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin.
Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GART Recombinant Rabbit Monoclonal Antibody [SC66-02] (ET1610-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, A431, Hela, HepG2, mouse uterus tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC66-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

107 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GART

SYNONYMS

5'-phosphoribosylglycinamide transformylase antibody; AIR synthase antibody; AIRS antibody; GAR transformylase antibody; GARS antibody; GART antibody; GARTF antibody; Glycinamide ribonucleotide synthetase antibody; MGC47764 antibody; PAIS antibody; PGFT antibody; Phosphoribosyl-aminoimidazole synthetase antibody; Phosphoribosylglycinamide formyltransferase antibody; Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody; Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29 antibody; Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase antibody; Phosphoribosylglycinamide synthetase antibody; PRGS antibody; PUR2_HUMAN antibody; Trifunctional purine biosynthetic protein adenosine 3 antibody

SEQUENCE SIMILARITIES

In the N-terminal section; belongs to the GARS family.; In the central section; belongs to the AIR synthase family.; In the C-terminal section; belongs to the GART family.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Membrane.

FUNCTION

Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events.