PRODUCT CODE: ER40201

GAP43 Antibody (ER40201)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain<br />
 Lane 2: Mouse brain<br />
 Lane 3: Mouse heart<br />
 Lane 4: Human skeletal muscle<br />
 Lane 5: N2A<br />
 Lane 6: A172<br />
 Lane 7: Human heart
  • Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain<br />
 Lane 2: Mouse brain<br />
 Lane 3: Mouse heart<br />
 Lane 4: Human skeletal muscle<br />
 Lane 5: N2A<br />
 Lane 6: A172<br />
 Lane 7: Human heart
  • ICC staining GAP43 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GAP43 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GAP43 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GAP43 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.
Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: Mouse brain
Lane 3: Mouse heart
Lane 4: Human skeletal muscle
Lane 5: N2A
Lane 6: A172
Lane 7: Human heart

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GAP43 Antibody (ER40201)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, NIH/3T3, N2A, A172, SHG-44, mouse brain tissue, rat brain tissue, human skeletal muscle tissue, human heart tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

GAP43

SYNONYMS

Axonal membrane protein GAP 43 antibody; Axonal membrane protein GAP-43 antibody; B 50 antibody; Calmodulin binding protein P 57 antibody; F1 antibody; GAP 43 antibody; GAP43 antibody; Growth Associated Protein 43 antibody; Growth-associated protein 43 antibody; Nerve Growth Related Peptide antibody; Nerve growth related peptide GAP43 antibody; NEUM_HUMAN antibody; Neural phosphoprotein B 50 antibody; Neural phosphoprotein B-50 antibody; Neuromodulin antibody; Neuron growth associated protein 43 antibody; PP46 antibody; Protein F1 antibody; QtrA-11580 antibody; QtrA-13071 antibody

SEQUENCE SIMILARITIES

Belongs to the neuromodulin family.

TISSUE SPECIFICITY

Expressed in the hippocampus (at protein level). Expressed in the dorsal root ganglion and the spinal cord (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).; Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 (By similarity).

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.

CITATIONS

  • Zhang, Fanrong et al.

    GAP43, a novel metastasis promoter in non-small cell lung cancer. | Journal of Translational Medicine [2018]