Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
GADD34 Monoclonal Antibody (EM1902-26)
Immunogen
Synthetic peptide corresponding to n terminal of human gadd34.
Host
Mouse
Positive Control
HepG2 cell lysate, K562 cell lysate, human prostate carcinoma tissue, K562.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A4D7
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
73 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:1,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
GADD34
SYNONYMS
Apoptosis associated protein antibody; GADD 34 antibody; Growth arrest and DNA damage inducible 34 antibody; Growth arrest and DNA damage-inducible protein GADD34 antibody; Myeloid differentiation primary response protein MyD116 homolog antibody; Ppp1r15a antibody; PR15A_HUMAN antibody; Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody; Protein phosphatase 1 regulatory subunit 15A antibody
SEQUENCE SIMILARITIES
Belongs to the PPP1R15 family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2.; Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Mitochondrion outer membrane.
FUNCTION
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.