PRODUCT CODE: ER1802-33

Fusion glycoprotein F0 Antibody (ER1802-33)

Applications

  • WB

  • ELISA

REACTIVITY

  • Avian avulavirus 1

Western blot analysis of Fusion glycoprotein F0 on Fusion glycoprotein F0 transfected HEK293 cell lysates using anti- Fusion glycoprotein F0 antibody at 1/500 dilution.
  • Western blot analysis of Fusion glycoprotein F0 on Fusion glycoprotein F0 transfected HEK293 cell lysates using anti- Fusion glycoprotein F0 antibody at 1/500 dilution.
Western blot analysis of Fusion glycoprotein F0 on Fusion glycoprotein F0 transfected HEK293 cell lysates using anti- Fusion glycoprotein F0 antibody at 1/500 dilution.

Applications

  • WB

  • ELISA

REACTIVITY

  • Avian avulavirus 1

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Fusion glycoprotein F0 Antibody (ER1802-33)

Immunogen

Peptide

Host

Rabbit

Positive Control

Fusion glycoprotein F0 transfected HEK293 cell.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1,000

  • ELISA

  • 1:5,000-1:10,000

TARGET

UNIPROT #

PROTEIN NAME

Fusion glycoprotein F0

SYNONYMS

F antibody; Fusion glycoprotein F0 antibody; Protein F antibody; RSV Fusion (F) Glycoprotein antibody

SEQUENCE SIMILARITIES

Belongs to the paramyxoviruses fusion glycoprotein family.

SUBCELLULAR LOCATION

Host cell membrane. Virion membrane.

FUNCTION

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.