PRODUCT CODE: R1511-25

FPR2 Rabbit Polyclonal Antibody (R1511-25)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis on different lysates using anti-FPR2 rabbit polyclonal antibody. Positive control: <br />
Lane 1: Jurkat     <br />
Lane 2: HL-60
  • Western blot analysis on different lysates using anti-FPR2 rabbit polyclonal antibody. Positive control: <br />
Lane 1: Jurkat     <br />
Lane 2: HL-60
  • Immunocytochemical staining of Hela cells using anti-FPR2 rabbit polyclonal antibody.
  • Immunocytochemical staining of PC-3M cells using anti-FPR2 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-FPR2 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-FPR2 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-FPR2 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-FPR2 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-FPR2 rabbit polyclonal antibody.
  • Flow cytometric analysis of Jurkat cells with FPR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis on different lysates using anti-FPR2 rabbit polyclonal antibody. Positive control:
Lane 1: Jurkat
Lane 2: HL-60

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FPR2 Rabbit Polyclonal Antibody (R1511-25)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, Jurkat, PC-3M, HL-60, mouse lung tissue, human lung tissue, mouse testis tissue, human spleen tissue, mouse spleen tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

50kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FPR2

SYNONYMS

ALXR antibody; FMLP-R-I antibody; FMLP-R-II antibody; FMLP-related receptor I antibody; FMLPX antibody; Formyl peptide receptor 2 antibody; Formyl peptide receptor related antibody; Formyl peptide receptor-like 1 antibody; FPR2 antibody; FPR2_HUMAN antibody; FPR2A antibody; FPRH1 antibody; FPRH2 antibody; FPRL1 antibody; HM63 antibody; Lipoxin A4 receptor antibody; LXA4 receptor antibody; LXA4R antibody; N-formyl peptide receptor 2 antibody; RFP antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

The N-formyl peptide receptor (FPR) family is comprised of three members, FPR, FPR like-1 (FPRL1, also designated lipoxin A4 receptor, FPRH1 and FPR2) and FPR like-2 (FPRL2), all of which are chemotactic G protein-coupled receptors that contain seven transmembrane domains. These receptors are found on the surface of phagocytic leukocytes, such as neutrophils and monocytes, and each family member contains specific residues, which are responsible for determining its ligand specificity. FPRL1 is a promiscuous receptor that binds to several ligands, including lipoxin A4, N-formyl-methionyl-leucyl-phenylalanine (fMLP), serum amyloid A (SAA), prion peptide and the 42 amino acid form of b amyloid. Upon activation, FPRL1 induces migration and calcium mobilization in human monocytes and neutrophils and is involved in inflammatory and host defense responses. FPRL1 may mediate inflammation in prion and Alzheimer’s diseases, which makes it a potential target for therapeutic agents.