FOXP3 Recombinant Rabbit Monoclonal Antibody [PSH07-47] (HA722835)
Catalog# HA722835
Rabbit Monoclonal to FOXP3
Tested for
-
WB
-
IHC-P
-
mIHC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of FOXP3 on different lysates with Rabbit anti-FOXP3 antibody (HA722835) at 1/1,000 dilution.
Lane 1: Mouse thymus tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat spleen tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722835) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FOXP3 on different lysates with Rabbit anti-FOXP3 antibody (HA722835) at 1/1,000 dilution.
Lane 1: Mouse thymus tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat spleen tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722835) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.