PRODUCT CODE: ER1802-95

Fibulin 5 Antibody (ER1802-95)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
  • Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
  • ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Fibulin 5 Antibody (ER1802-95)

Immunogen

Recombinant protein within human fibulin 5 210-360 aa.

Host

Rabbit

Positive Control

Human skin tissue, JAR, SiHa, SKOV-3, rat testis tissue, mouse ovary tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Fibulin-5

GENE NAME

FBLN5

SYNONYMS

FIBL-5, Dance, UP50, FBLN5, DANCE

SEQUENCE SIMILARITIES

Belongs to the fibulin family.

TISSUE SPECIFICITY

Expressed in skin fibroblasts (at protein level)(PubMed:17035250). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Secreted. Secreted, extracellular space, extracellular matrix. Note=co-localizes with ELN in elastic fibers.

FUNCTION

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN.