PRODUCT CODE: HA1031

Fc region of Rabbit IgG Recombinant Alpaca Monoclonal Antibody (HA1031)

  • Nanobody
  • Recombinant

Applications

  • IP

  • ELISA

  • IHC

  • WB

REACTIVITY

  • Rabbit

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Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).<br />
<br />
Exposure time: 30 seconds
  • Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).<br />
<br />
Exposure time: 30 seconds
  • The Cross-reactions of the secondary antibody (HA1031) with Mouse IgG and Human IgG.<br />
<br />
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:80,000 (Lane A, C) and 1:160,000 (Lane B, D).<br />
<br />
Lane A, B: Mouse IgG (30 ng per lane).<br />
Lane C, D: Human IgG (30 ng per lane).<br />
<br />
Exposure time: 2 minutes
  • Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody.<br />
<br />
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.<br />
<br />
The inset negative control image is secondary-only at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.<br />
<br />
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).

Exposure time: 30 seconds

Applications

  • IP

  • ELISA

  • IHC

  • WB

REACTIVITY

  • Rabbit

SPECIFICATIONS

Product Type

Recombinant Alpaca secondary

Product Name

Fc region of Rabbit IgG Recombinant Alpaca Monoclonal Antibody (HA1031)

Immunogen

Fc region of rabbit igg.

Host

Alpaca

Conjugation

Hrp

Clonality

Monoclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.5% BSA, 50% Glycerol.

Concentration

1 ug/ul

PURIFICATION

Antigen affinity purified.

MOLECULAR WEIGHT

15 kDa

Isotype

IgG

APPLICATION DILUTION

  • IP

  • assay-dependent

  • ELISA

  • 1:5,000-1:20,000

  • IHC

  • 1:100-1:500

  • WB

  • 1:10,000-1:100,000

TARGET

PROTEIN NAME

Fc region of Rabbit IgG