PRODUCT CODE: ER1706-78

FBXW7 Antibody (ER1706-78)

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse spleen tissue <br />
 Lane 2: Rat lung tissue<br />
 Lane 3: HepG2<br />
  • Western blot analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse spleen tissue <br />
 Lane 2: Rat lung tissue<br />
 Lane 3: HepG2<br />
  • ICC staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FBXW7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse spleen tissue
Lane 2: Rat lung tissue
Lane 3: HepG2

Applications

  • WB

  • ICC

  • FC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FBXW7 Antibody (ER1706-78)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, rat lung tissue lysate, HepG2, Hela, HUVEC, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

F-box/WD repeat-containing protein 7

GENE NAME

FBXW7

SYNONYMS

hAgo, FBXW7, FBW7

TISSUE SPECIFICITY

Isoform 1 is widely expressed. Isoform 3 is expressed in brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation.; Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its proteasomal degradation.

SUBCELLULAR LOCATION

[Isoform 1]: Nucleus, nucleoplasm.; [Isoform 2]: Cytoplasm.; [Isoform 3]: Nucleus, nucleolus.

FUNCTION

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination.