PRODUCT CODE: ER1802-89

FBXL18 Rabbit Polyclonal Antibody (ER1802-89)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of FBXL18 on PC-3M cell lysate using anti-FBXL18 antibody at 1/500 dilution.
  • Western blot analysis of FBXL18 on PC-3M cell lysate using anti-FBXL18 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-FBXL18 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with FBXL18 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of FBXL18 on PC-3M cell lysate using anti-FBXL18 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FBXL18 Rabbit Polyclonal Antibody (ER1802-89)

Immunogen

Synthetic peptide within human fbxl18 aa 756-805 / 805.

Host

Rabbit

Positive Control

PC-3M, rat liver tissue, human liver tissue, mouse liver tissue, A549.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

88/40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FBXL18

SYNONYMS

F box and leucine rich repeat protein 18 antibody; F box/LRR repeat protein 18 antibody; FBL18 antibody; FBXL 18 antibody; FLJ10776 antibody; FLJ11467 antibody; FLJ26934 antibody; FLJ38075 antibody; FLJ41541 antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

FBL18 (F-box and leucine-rich repeat protein 18), also known as FBXL18, is an 805 amino acid protein that contains an F-box near its N-terminus, followed by several leucine-rich repeats and a transmembrane domain at the C-terminus. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins and cyclin-dependent kinase inhibitors (CDKNs), for degradation by the proteasome after ubiquitination. FBL18 directly interacts with SKP1A p19 and CUL-1, forming a substrate-recognition component of the SCF-type E3 ubiquitin ligase complex. Four isoforms of FBL18 exist due to alternative splicing.