PRODUCT CODE: ER1706-84

FAP Antibody (ER1706-84)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of FAP on Siha cell lysate using anti-FAP antibody at 1/1,000 dilution.
  • Western blot analysis of FAP on Siha cell lysate using anti-FAP antibody at 1/1,000 dilution.
  • ICC staining FAP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining FAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of SH-SY5Y cells with FAP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of FAP on Siha cell lysate using anti-FAP antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

FAP Antibody (ER1706-84)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, NIH-3T3, Siha, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

FAP

SYNONYMS

Prolyl endopeptidase FAP antibody; 170 kDa melanoma membrane-bound gelatinase antibody; Dipeptidyl peptidase FAP antibody; Fibroblast activation protein alpha antibody; FAPalpha antibody; Gelatine degradation protease FAP antibody; Integral membrane serine protease antibody; Post-proline cleaving enzyme antibody; Serine integral membrane protease antibody; SIMP antibody; Surface-expressed protease antibody; Seprase antibody; FAP antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S9B family.

TISSUE SPECIFICITY

Expressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from inflamed synovial tissues. Expressed in activated hepatic stellate cells (HSC) and myofibroblasts from cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (at protein level). Expressed in glioblastomas and glioma cells. Isoform 1 and isoform 2 are expressed in melanoma, carcinoma and fibroblast cell lines.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; The N-terminus may be blocked.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.