Lane 1: C2C12 cell lysate
Lane 2: HepG2 cell lysate
Rabbit polyclonal primary
ERN1 Antibody (ER1902-90)
Synthetic peptide within n-terminal human ern1.
C2C12 cell lysate, HepG2 cell lysate, rat uterus tissue, human appendix tissue.
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified.
Predicted band size 110 kDa.
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody; Endoplasmic reticulum to nucleus signaling 1 antibody; Endoplasmic reticulum-to-nucleus signaling 1 antibody; Endoribonuclease antibody; ER to nucleus signaling 1 antibody; ERN 1 antibody; Ern1 antibody; ERN1_HUMAN antibody; hIRE 1p antibody; hIRE1p antibody; Inositol requiring 1 antibody; Inositol requiring 1, S. cerevisiae, homolog of antibody; Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody; Inositol requiring protein 1 antibody; inositol-requiring enzyme 1 antibody; Inositol-requiring protein 1 antibody; IRE 1 antibody; IRE 1a antibody; IRE 1P antibody; Ire1 alpha antibody; Ire1-alpha antibody; IRE1a antibody; Ire1alpha antibody; IRE1P antibody; MGC163277 antibody; MGC163279 antibody; Protein kinase/endoribonuclease antibody; RGD1559716 antibody; Serine/threonine protein kinase/endoribonuclease IRE1 antibody
Endoplasmic reticulum, Membrane.
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity . The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA . The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A .