PRODUCT CODE: em1111

EP-CAM Monoclonal Antibody (EM1111)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution. <br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: NIH/3T3<br />
Lane 3: MCF-7
  • Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution. <br />
Positive control:<br />
Lane 1: Hela<br />
Lane 2: NIH/3T3<br />
Lane 3: MCF-7
  • ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
  • ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: MCF-7

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

EP-CAM Monoclonal Antibody (EM1111)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, NIH/3T3, MCF-7, A431, SW1990, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-E1-E2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein L purified.

MOLECULAR WEIGHT

35 kDa

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:100-1:200

  • IHC-P

  • 1:200-1:500

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Epithelial cell adhesion molecule

GENE NAME

EPCAM

SYNONYMS

Ep-CAM,EGP,EGP314,hEGP314

SEQUENCE SIMILARITIES

Belongs to the EPCAM family.

TISSUE SPECIFICITY

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.

POST-TRANSLATIONAL MODIFICATION

Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

SUBCELLULAR LOCATION

Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction.

FUNCTION

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.