PRODUCT CODE: ER1803-25

Eg5 Antibody (ER1803-25)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Eg5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Eg5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Eg5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Eg5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with Eg5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Eg5 Antibody (ER1803-25)

Immunogen

Recombinant protein within human eg5 aa 100-300.

Host

Rabbit

Positive Control

A431, SH-SY-5Y, SiHa, rat kidney tissue, human lung cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

119 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Eg5

SYNONYMS

EG5 antibody; HKSP antibody; KIF11 antibody; KIF11_HUMAN antibody; Kinesin family member 11 antibody; Kinesin like protein 1 antibody; Kinesin-like protein 1 antibody; Kinesin-like protein KIF11 antibody; Kinesin-like spindle protein HKSP antibody; Kinesin-related motor protein Eg5 antibody; KNSL1 antibody; MCLMR antibody; Thyroid receptor-interacting protein 5 antibody; TR-interacting protein 5 antibody; TRIP-5 antibody; TRIP5 antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

SUBCELLULAR LOCATION

Cytoplasm, Cytoskeleton.

FUNCTION

Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.