PRODUCT CODE: EM50104

E tag Monoclonal Antibody (EM50104)

Applications

  • WB

  • ELISA

  • IP

  • ICC

REACTIVITY

Western blot analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20000 dilution.
  • Western blot analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20000 dilution.
Western blot analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20000 dilution.

Applications

  • WB

  • ELISA

  • IP

  • ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

E tag Monoclonal Antibody (EM50104)

Immunogen

Peptide

Host

Mouse

Positive Control

E tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B2-E2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:20,000

TARGET

PROTEIN NAME

E tag

SYNONYMS

E epitope tag antibody; GAPVPYPDPLEPR epitope tag antibody; GAPVPYPDPLEPR tag antibody

FUNCTION

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. E-tag is an epitope tag composed of a 13 residue peptide. This antibody recognizes the E-tag fused to either the amino- or carboxy-terminus of targeted proteins.

CITATIONS

  • Zhao, Liangzhong et al.

    Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1�. | Scientific Reports [2019]