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PRODUCT CODE: ER1511-12

Dysferlin (DYSF) Antibody (ER1511-12)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Dysferlin on human fetal skeletal muscle tissue lysate using anti-Dysferlin antibody at 1/500 dilution.
  • Western blot analysis of Dysferlin on human fetal skeletal muscle tissue lysate using anti-Dysferlin antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HUVEC cells with Dysferlin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Dysferlin on human fetal skeletal muscle tissue lysate using anti-Dysferlin antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Dysferlin (DYSF) Antibody (ER1511-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human fetal skeletal muscle tissue, human kidney tissue, human placenta tissue, HUVEC.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

237 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O75923

PROTEIN NAME

Dysferlin (DYSF)

SYNONYMS

DMAT antibody; DYSF antibody; DYSF_HUMAN antibody; Dysferlin antibody; Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) antibody; Dysferlin limb girdle muscular dystrophy 2B antibody; Dystrophy associated fer 1 like 1 antibody; Dystrophy associated fer 1 like protein antibody; Dystrophy associated fer1 like 1 antibody; Dystrophy associated fer1 like protein antibody; Dystrophy-associated fer-1-like protein antibody; Fer 1 like protein 1 antibody; Fer-1-like protein 1 antibody; Fer1 like protein 1 antibody; FER1L1 antibody; FLJ00175 antibody; FLJ90168 antibody; LGMD 2B antibody; LGMD2B antibody; Limb girdle muscular dystrophy 2B (autosomal recessive) antibody; Limb girdle muscular dystrophy 2B antibody; Miyoshi myopathy antibody; MM antibody; MMD1 antibody

SEQUENCE SIMILARITIES

Belongs to the ferlin family.

TISSUE SPECIFICITY

Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Ubiquitous. Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas.

DEVELOPMENTAL STAGE

Expression in limb tissue from 5-6 weeks embryos; persists throughout development.

SUBCELLULAR LOCATION

Plasma membrane. Cytoplasmic vesicle.

FUNCTION

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.