PRODUCT CODE: ER2001-60

Dux Antibody (ER2001-60)

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Non-transfected 293T cells lysate<br />
Lane 2: His-tagged Dux transfected 293T cells lysate
  • Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Non-transfected 293T cells lysate<br />
Lane 2: His-tagged Dux transfected 293T cells lysate
  • ICC staining of Dux in Non-transfected 293T and His-tagged Dux transfected 293T cells cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Non-transfected 293T cells lysate
Lane 2: His-tagged Dux transfected 293T cells lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Dux Antibody (ER2001-60)

Immunogen

Synthetic peptide within mouse dux aa 520-570.

Host

Rabbit

Positive Control

Non-transfected 293T cells lysate, His-tagged Dux transfected 293T cells lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

74 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Homeobox protein Dux

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The double homeobox (DUX) proteins are encoded by 3.3-kilobase repeats found throughout the human genome. The DUX family includes DUX1, DUX2, DUX3, DUX4 and DUX5. Each of these family members, excluding DUX2, contains two homeobox domains. DUX2 contains only one homeobox domain. DUX1 and DUX5 are identical to one another and they share 98% identity with DUX3 and approximately 70% identity with DUX2. The genes encoding DUX5 and DUX3 both contain additional start sites that result in N-terminal extended isoforms. The homeodomains found in DUX5 and DUX1 are similar to those found in Pax-3, Pax-7, OTX1 and OTX2. DUX4, also known as DUX10, is capable of forming homodimers. In addition, the gene encoding DUX4 maps within the D4Z4 repeat unit that has been implicated in Facioscapulohumeral muscular dystrophy (FSHD).