PRODUCT CODE: 0903-3

DOPEY2 Antibody (0903-3)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.
  • Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.
  • ICC staining Dopey-2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin.
  • Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DOPEY2 Antibody (0903-3)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse heart tissue lysate, human heart tissue lysate, D3 cells, N2A cells, mouse heart tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

260kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein dopey-2

GENE NAME

DOP1B

SYNONYMS

DOPEY2

SEQUENCE SIMILARITIES

Belongs to the dopey family.

TISSUE SPECIFICITY

Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients.

DEVELOPMENTAL STAGE

Abundantly expressed in developing central nervous system, with highest levels in cerebellum and lowest in telencephalon.

SUBCELLULAR LOCATION

Golgi apparatus membrane; Peripheral membrane protein.

FUNCTION

May be involved in protein traffic between late Golgi and early endosomes.