PRODUCT CODE: ER1901-30

DOG1 Antibody (ER1901-30)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of DOG1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DOG1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of DOG1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of DOG1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-DOG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human seminal pouch tissue using anti-DOG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of DOG1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of DOG1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DOG1 Antibody (ER1901-30)

Immunogen

Synthetic peptide within c-terminal human cd43.

Host

Rabbit

Positive Control

PC-3M cell lysates, A549, HepG2, human liver carcinoma tissue, human seminal pouch tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

114/97 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1000

  • ICC:1:50-1:200

  • IHC-P:1:50-1:200

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DOG1

SYNONYMS

ANO 1 antibody; ANO1 antibody; ANO1_HUMAN antibody; Anoctamin 1 antibody; Anoctamin 1 calcium activated chloride channel antibody; Anoctamin-1 antibody; Anoctamin1 antibody; Ca2+ activated Cl- channel antibody; Calcium Activated Chloride Channel antibody; Discovered on gastrointestinal stromal tumors protein 1 antibody; DOG 1 antibody; DOG1 antibody; FLJ10261 antibody; Membrane protein antibody; Oral cancer overexpressed 2 antibody; Oral cancer overexpressed protein 2 antibody; ORAOV 2 antibody; ORAOV2 antibody; TAOS 2 antibody; TAOS2 antibody; TMEM 16A antibody; TMEM16A antibody; Transmembrane protein 16A (eight membrane spanning domains) antibody; Transmembrane protein 16A antibody; Tumor amplified and overexpressed sequence 2 antibody; Tumor-amplified and overexpressed sequence 2 antibody

SEQUENCE SIMILARITIES

Belongs to the anoctamin family.

TISSUE SPECIFICITY

Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles.

SUBCELLULAR LOCATION

Cell membrane, cytoplasm.

FUNCTION

ANO1 (anoctamin 1), also known as DOG1, ORAOV2, TAOS2 or TMEM16A, is a 986 amino acid multi-pass membrane protein that localizes to both the cell membrane and the cytoplasm and belongs to the anoctamin family. Expressed in a variety of tissues with highest expression in liver, gastrointestinal muscle and skeletal muscle, ANO1 functions as a calcium-activated chloride channel that is required for normal tracheal development. Human ANO1 shares 90% sequence identity with its mouse counterpart, suggesting a conserved role between species. ANO1 is present in breast, pancreatic, gastric, and uterine cancers, as well as in neck, ovarian and parathyroid tumors, suggesting a role for ANO1 in carcinogenesis. Three isoforms of ANO1 exist due to alternative splicing events.