PRODUCT CODE: ER1802-55

Dnmt3b Antibody (ER1802-55)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Dnmt3b on K562 cell lysate using anti-Dnmt3b antibody at 1/500 dilution.
  • Western blot analysis of Dnmt3b on K562 cell lysate using anti-Dnmt3b antibody at 1/500 dilution.
  • ICC staining Dnmt3b in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with Dnmt3b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Dnmt3b on K562 cell lysate using anti-Dnmt3b antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Dnmt3b Antibody (ER1802-55)

Immunogen

Synthetic peptide within human dnmt3b aa 1-50.

Host

Rabbit

Positive Control

K562, 293T, mouse testis tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DNA

GENE NAME

DNMT3B

SYNONYMS

Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B

SEQUENCE SIMILARITIES

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

TISSUE SPECIFICITY

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

POST-TRANSLATIONAL MODIFICATION

Sumoylated.; Citrullinated by PADI4.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.