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PRODUCT CODE: ER1512-20

DKK1 Antibody (ER1512-20)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse brain <br /> Lane 2: Human fetal brain<br />
  • Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Mouse brain <br /> Lane 2: Human fetal brain<br />
  • ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: Human fetal brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DKK1 Antibody (ER1512-20)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse brain tissue lysate, human fetal brain tissue lysate, Hela, NCCIT.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O94907

PROTEIN NAME

Dickkopf-related protein 1

GENE NAME

DKK1

SYNONYMS

Dickkopf-1, Dkk-1, hDkk-1

SEQUENCE SIMILARITIES

Belongs to the dickkopf family.

TISSUE SPECIFICITY

Placenta.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).