PRODUCT CODE: ER1802-54

DIAPH3 Antibody (ER1802-54)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
  • Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
  • ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DIAPH3 Antibody (ER1802-54)

Immunogen

Synthetic peptide within human diaph3 aa 1-50.

Host

Rabbit

Positive Control

A431, LOVO, SiHa, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

137/80 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

DIAPH3

SYNONYMS

AN antibody; AUNA1 antibody; Dia2 antibody; diap3 antibody; DIAP3_HUMAN antibody; DIAPH3 antibody; Diaphanous homolog 3 (Drosophila) antibody; Diaphanous homolog 3 antibody; Diaphanous related formin 3 antibody; Diaphanous, Drosophila, homolog of, 3 antibody; Diaphanous-related formin-3 antibody; DKFZp434C0931 antibody; DKFZp686A13178 antibody; DRF3 antibody; FLJ34705 antibody; mDia2 antibody; NSDAN antibody; OTTHUMP00000018480 antibody; Protein diaphanous homolog 3 antibody; RP11-26P21.1 antibody

SEQUENCE SIMILARITIES

Belongs to the formin homology family. Diaphanous subfamily.

DEVELOPMENTAL STAGE

Increased expression in S phase and mitotic cells; levels decrease as cells enter in G0/G1 phase due to proteasomal degradation (at protein level).

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

DIAPH3 (diaphanous homolog 3), also known as DIAP3, DRF3 or mDia2 of mouse origin, is a 1,193 amino acid member of the formin homology protein family and is required for the correct function of various cellular processes. DIAPH3 binds to both Profilin, a protein involved in cell maintenance, and to the GTP-bound form of Rho (Rho-GTP). Binding to both of these proteins allows DIAPH3 to recruit Profilin to the membrane, in a Rho-dependent manner. At the membrane, DIAPH3 promotes Actin polymerization and is required for stress fiber formation, cytokinesis and transcriptional activation of the serum response factor (SRF). DIAPH3 also regulates Actin dynamics by coupling Src tyrosine kinase (c-Src) and Rho during Actin signaling events. DIAPH3 contains one diaph-anous autoregulatory domain (DAD) and one Rho GTPase-binding domain (GBD). When DAD and GBD are intramolecularly bound, the GBD is occupied and DIAPH3 is inactive. Interruption of the DAD-GBD bond allows the GBD to bind to Rho-GTP, thus activating DIAPH3. Seven isoforms of DIAPH3 exist due to alternative splicing events.