PRODUCT CODE: ER1802-54

DIAPH3 Antibody (ER1802-54)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
  • Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
  • ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DIAPH3 Antibody (ER1802-54)

Immunogen

Synthetic peptide within human diaph3 aa 1-50.

Host

Rabbit

Positive Control

A431, LOVO, SiHa, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

137/80 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Protein diaphanous homolog 3

GENE NAME

DIAPH3

SYNONYMS

DRF3, DIAPH3, DIAP3

SEQUENCE SIMILARITIES

Belongs to the formin homology family. Diaphanous subfamily.

DEVELOPMENTAL STAGE

Increased expression in S phase and mitotic cells; levels decrease as cells enter in G0/G1 phase due to proteasomal degradation (at protein level).

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.