Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Mouse monoclonal primary
DFNA5/GSDME Monoclonal Antibody (EM1901-49)
Synthetic peptide within human dfna5 aa 30-230.
Hela cell lysates, HepG2 cell lysates, A431, SiHa, SH-SY5Y.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein G affinity purified.
Belongs to the gasdermin family.
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) relieves autoinhibition and is sufficient to initiate pyroptosis.
[Gasdermin-E, N-terminal]: Cell membrane.; [Gasdermin-E]: Cytoplasm, cytosol.
Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.; [Gasdermin-E, N-terminal]: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity.