PRODUCT CODE: ER40113

Desmin Antibody (ER40113)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

Western blot analysis of Desmin on different cell lysates using anti-Desmin antibody at 1/2000 dilution.<br />
 Positive control:<br />
 Lane 1: Human skeletal muscle tissue<br />
 Lane 2: Mouse heart tissue<br />
 Lane 3: Human heart tissue
  • Western blot analysis of Desmin on different cell lysates using anti-Desmin antibody at 1/2000 dilution.<br />
 Positive control:<br />
 Lane 1: Human skeletal muscle tissue<br />
 Lane 2: Mouse heart tissue<br />
 Lane 3: Human heart tissue
  • ICC staining of Desmin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Desmin in NIH-3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Desmin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin.
  • Western blot analysis of Desmin on hybrid fish (crucian-carp) brain tissue lysate using anti-Desmin antibody at 1/500 dilution.
Western blot analysis of Desmin on different cell lysates using anti-Desmin antibody at 1/2000 dilution.
Positive control:
Lane 1: Human skeletal muscle tissue
Lane 2: Mouse heart tissue
Lane 3: Human heart tissue

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Desmin Antibody (ER40113)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human heart tissue, human skeletal muscle tissue, mouse heart tissue, human uterus tissue, mouse skeletal muscle tissue, HepG2,NIH-3T3,Hela

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Desmin

SYNONYMS

CMD1I antibody; CSM1 antibody; CSM2 antibody; DES antibody; DESM_HUMAN antibody; Desmin antibody; FLJ12025 antibody; FLJ39719 antibody; FLJ41013 antibody; FLJ41793 antibody; Intermediate filament protein antibody; OTTHUMP00000064865 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

POST-TRANSLATIONAL MODIFICATION

ADP-ribosylation prevents ability to form intermediate filaments.; Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Desmin is one of the earliest protein markers for muscle tissue in embryogenesis as it is detected in the somites. Although it is present early in the development of muscle cells, it is only expressed at low levels, and increases as the cell nears terminal differentiation. Desmin is also important in muscle cell architecture and structure since it connects many components of the cytoplasm. Finally, desmin may be important in mitochondria function. Desmin-related myopathy (DRM or Desminopathy) is a subgroup of the myofibrillar myopathy diseases and is the result of a mutation in the gene that codes for desmin which prevents it from forming protein filaments, instead forming aggregates of desmin and other proteins throughout the cell. Recently, mutations were identified in patients suffered by an arrhythmogenic right ventricular cardiomyopathy (ARVC).