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PRODUCT CODE: EM1902-03

ddx24 Monoclonal Antibody (EM1902-03)

  • Zebrafish

Applications

  • IHC-P

REACTIVITY

  • Zebrafish

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Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-ddx24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-03, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-ddx24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-03, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-ddx24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-03, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • IHC-P

REACTIVITY

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ddx24 Monoclonal Antibody (EM1902-03)

Immunogen

Recombinant protein within zebrafish ddx24 aa 550-820.

Host

Mouse

Positive Control

Zebrafish tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3B10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

94 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • IHC-P:1:100-1:500

TARGET

UNIPROT #

A0A0R4IA19

PROTEIN NAME

ddx24

SYNONYMS

DEAD (Asp-Glu-Ala-Asp) box helicase 24; ddx24

SUBCELLULAR LOCATION

Nucleolus.

FUNCTION

ATP-dependent RNA helicase DDX24 is an enzyme that in humans is encoded by the DDX24 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial RNA splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level.