Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
DDIT4 Rabbit Polyclonal Antibody (ER1706-76)
Immunogen
Peptide.
Host
Rabbit
Positive Control
Hela and K562 cell lysates, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, A549.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
25 kDa (Predicted band size)
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
ICC
-
1:50-1:100
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
DDIT4
SYNONYMS
DDIT4 antibody; DDIT4_HUMAN antibody; Dig2 antibody; DNA damage inducible transcript 4 antibody; DNA damage inducible transcript 4 protein antibody; DNA damage-inducible transcript 4 protein antibody; FLJ20500 antibody; HIF 1 responsive protein RTP801 antibody; HIF 1 responsive RTP801 antibody; HIF-1 responsive protein RTP801 antibody; Protein regulated in development and DNA damage response 1 antibody; REDD-1 antibody; REDD1 antibody; RTP801 antibody
SEQUENCE SIMILARITIES
Belongs to the DDIT4 family.
TISSUE SPECIFICITY
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by GSK3B; this promotes proteasomal degradation.; Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.
SUBCELLULAR LOCATION
Mitochondrion. Cytosol.
FUNCTION
REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.