PRODUCT CODE: ER1706-76

DDIT4 Antibody (ER1706-76)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.
  • Western blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.
  • ICC staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDIT4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with DDIT4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DDIT4 Antibody (ER1706-76)

Immunogen

Peptide.

Host

Rabbit

Positive Control

Hela and K562 cell lysates, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, A549.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

25 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DDIT4

SYNONYMS

DDIT4 antibody; DDIT4_HUMAN antibody; Dig2 antibody; DNA damage inducible transcript 4 antibody; DNA damage inducible transcript 4 protein antibody; DNA damage-inducible transcript 4 protein antibody; FLJ20500 antibody; HIF 1 responsive protein RTP801 antibody; HIF 1 responsive RTP801 antibody; HIF-1 responsive protein RTP801 antibody; Protein regulated in development and DNA damage response 1 antibody; REDD-1 antibody; REDD1 antibody; RTP801 antibody

SEQUENCE SIMILARITIES

Belongs to the DDIT4 family.

TISSUE SPECIFICITY

Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by GSK3B; this promotes proteasomal degradation.; Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.

SUBCELLULAR LOCATION

Mitochondrion. Cytosol.

FUNCTION

REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.