PRODUCT CODE: ER1803-20

DDIT3 Antibody (ER1803-20)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis tissue<br />
  Lane 2: LOVO<br />
  Lane 3: Mouse brain tissue
  • Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis tissue<br />
  Lane 2: LOVO<br />
  Lane 3: Mouse brain tissue
  • Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: LOVO
Lane 3: Mouse brain tissue

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DDIT3 Antibody (ER1803-20)

Immunogen

Synthetic peptide within human ddit3 aa 70-110.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, LOVO, mouse testis tissue lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DDIT3

SYNONYMS

C/EBP homologous protein antibody; C/EBP Homology Protein antibody; C/EBP zeta antibody; C/EBP-homologous protein 10 antibody; C/EBP-homologous protein antibody; CCAAT/enhancer binding protein homologous protein antibody; CEBPZ antibody; CHOP 10 antibody; CHOP antibody; CHOP-10 antibody; CHOP10 antibody; DDIT 3 antibody; DDIT-3 antibody; Ddit3 antibody; DDIT3_HUMAN antibody; DNA Damage Inducible Transcript 3 antibody; DNA damage-inducible transcript 3 protein antibody; GADD 153 antibody; GADD153 antibody; Growth Arrest and DNA Damage Inducible Protein 153 antibody; Growth arrest and DNA damage inducible protein GADD153 antibody; Growth arrest and DNA damage-inducible protein GADD153 antibody; MGC4154 antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its degradation by the proteasome.; Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

DDIT3 also named GADD 153 which has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.