PRODUCT CODE: ER1802-43

Cytokeratin 8 Antibody (ER1802-43)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Human small intestine<br />
  Lane 2: A431
  • Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Human small intestine<br />
  Lane 2: A431
  • ICC staining Cytokeratin 8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution.
Positive control:
Lane 1: Human small intestine
Lane 2: A431

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cytokeratin 8 Antibody (ER1802-43)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human small intestine tissue lysate, A431, A549, LOVO, MCF-7, rat epididymis tissue, human lung cancer tissue, human prostate tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin 8

SYNONYMS

CARD2 antibody; CK 8 antibody; CK-8 antibody; CK8 antibody; CYK8 antibody; CYKER antibody; Cytokeratin endo A antibody; Cytokeratin-8 antibody; DreK8 antibody; EndoA antibody; K2C8 antibody; K2C8_HUMAN antibody; K8 antibody; Keratin 8 antibody; Keratin type II cytoskeletal 8 antibody; Keratin, type II cytoskeletal 8 antibody; Keratin-8 antibody; KO antibody; Krt 2.8 antibody; KRT8 antibody; MGC118110 antibody; MGC174782 antibody; MGC53564 antibody; MGC85764 antibody; sb:cb186 antibody; Type-II keratin Kb8 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.; O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.; O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.