PRODUCT CODE: EM0702

Cytokeratin-7 Monoclonal Antibody (EM0702)

  • IVD–IHC

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution.<br />
 Positive control: <br />
  Lane 1: MCF-7 <br />
  Lane 2: A549 <br />
  Lane 3: Hela <br />
  Lane 4: HepG2 <br />
  Lane 5: SKBR3 Lane 6: SKOV3 <br />
  Lane 7: SW1990
  • Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution.<br />
 Positive control: <br />
  Lane 1: MCF-7 <br />
  Lane 2: A549 <br />
  Lane 3: Hela <br />
  Lane 4: HepG2 <br />
  Lane 5: SKBR3 Lane 6: SKOV3 <br />
  Lane 7: SW1990
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.
Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: A549
Lane 3: Hela
Lane 4: HepG2
Lane 5: SKBR3 Lane 6: SKOV3
Lane 7: SW1990

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Cytokeratin-7 Monoclonal Antibody (EM0702)

Immunogen

Peptide

Host

Mouse

Positive Control

MCF-7, A549, Hela, HepG2, SKBR3, SKOV3, SW1990, human lung?carcinoma tissue, human breast tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-G3-D8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Cytokeratin-7

SYNONYMS

CK 7 antibody; CK-7 antibody; CK7 antibody; Cytokeratin 7 antibody; Cytokeratin-7 antibody; D15Wsu77e antibody; K2C7 antibody; K2C7_HUMAN antibody; K7 antibody; Keratin 7 antibody; Keratin 7, type II antibody; Keratin type II cytoskeletal 7 antibody; Keratin, 55K type II cytoskeletal antibody; Keratin, simple epithelial antibody; Keratin, simple epithelial type I, K7 antibody; Keratin, type II cytoskeletal 7 antibody; Keratin-7 antibody; Krt2-7 antibody; KRT7 antibody; MGC11625 antibody; MGC129731 antibody; MGC3625 antibody; Sarcolectin antibody; SCL antibody; Type II mesothelial keratin K7 antibody; Type-II keratin Kb7 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

POST-TRANSLATIONAL MODIFICATION

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.