PRODUCT CODE: ER0722

Cyclin D1 Antibody (ER0722)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution.<br />
Positive control: mouse brain
  • Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution.<br />
Positive control: mouse brain
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin. <br />
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin. <br />
Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution.
Positive control: mouse brain

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cyclin D1 Antibody (ER0722)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse brain, human tonsil tissue, human liver carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

36kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

G1/S-specific cyclin-D1

GENE NAME

Ccnd1

SYNONYMS

CCND1

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin D subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).; Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner (By similarity). Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Nucleus membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

FUNCTION

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).