PRODUCT CODE: R1308-13

Cyclin B1 Antibody (R1308-13)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.
  • Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.
  • Immunofluorescent staining of F9 cells using anti- Cyclin B1 rabbit polyclonal antibody.
  • Immunofluorescent staining of Hela cells using anti- Cyclin B1 rabbit polyclonal antibody.
Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cyclin B1 Antibody (R1308-13)

Immunogen

Peptide

Host

Rabbit

Positive Control

293, Hela, F9

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

~58kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:500

TARGET

UNIPROT #

PROTEIN NAME

Cyclin B1

SYNONYMS

CCNB 1 antibody; CCNB antibody; ccnb1 antibody; CCNB1_HUMAN antibody; Cyclin B1 antibody; G2 mitotic specific cyclin B1 antibody; G2/mitotic-specific cyclin-B1 antibody

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin AB subfamily.

DEVELOPMENTAL STAGE

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.