Rabbit polyclonal primary
Cyclin B1 Antibody (R1308-13)
293, Hela, F9
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Immunogen affinity purified
CCNB 1 antibody; CCNB antibody; ccnb1 antibody; CCNB1_HUMAN antibody; Cyclin B1 antibody; G2 mitotic specific cyclin B1 antibody; G2/mitotic-specific cyclin-B1 antibody
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.