Cullin 2 Recombinant Rabbit Monoclonal Antibody [JG72-31] (ET7108-47)
Catalog# ET7108-47
Rabbit Monoclonal to Cullin 2
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: PC-3M cell lysate
Lane 2: K562 tissue lysate (20 µg/Lane)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 92 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-47) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cullin 2 Recombinant Rabbit Monoclonal Antibody [JG72-31] (ET7108-47)
Immunogen
Synthetic peptide within n-terminal human cullin 2 .
Host
Rabbit
Positive Control
PC-3M cell lysate, k562 tissue lysate, rat testis tissue, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG72-31
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
89 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cullin 2
SYNONYMS
Cul2 antibody;CUL 2 antibody;CUL-2 antibody;CUL2 antibody;CUL2_HUMAN antibody;cullin 2 antibody;cullin homolog 2 antibody;Cullin-2 antibody;MGC131970 antibody
SEQUENCE SIMILARITIES
Belongs to the cullin family.
POST-TRANSLATIONAL MODIFICATION
Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex.
CITATIONS
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Zhang Jian,et al
Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer
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WB
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Human
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Citation
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