PRODUCT CODE: em1701-84

CUB domain-containing protein 1 Monoclonal Antibody (EM1701-84)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CDCP1 on K562 cell lysates using anti-CDCP1 antibody at 1/200 dilution.
  • Western blot analysis of CDCP1 on K562 cell lysates using anti-CDCP1 antibody at 1/200 dilution.
  • ICC staining CDCP1 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CDCP1 (red) in A549 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.
Western blot analysis of CDCP1 on K562 cell lysates using anti-CDCP1 antibody at 1/200 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CUB domain-containing protein 1 Monoclonal Antibody (EM1701-84)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

A549, LOVO, K562, rat skeletal muscle tissue, human tonsil tissue, human colon cancer tissue, mouse colon tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B2-E6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

92/72/37 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50 IHC-P

TARGET

UNIPROT #

PROTEIN NAME

CUB domain-containing protein 1

GENE NAME

CDCP1

SYNONYMS

SIMA135, CD318, CDCP1, TRASK

TISSUE SPECIFICITY

Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.; N-glycosylated.; A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

SUBCELLULAR LOCATION

[Isoform 1]: Cell membrane; Single-pass membrane protein. Note=Shedding may also lead to a soluble peptide.; [Isoform 3]: Secreted.

FUNCTION

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.