PRODUCT CODE: ER1802-69

CTGF Antibody (ER1802-69)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.
  • Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.
  • ICC staining CTGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTGF in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTGF in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CTGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer tissue using anti-CTGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CTGF antibody. Counter stained with hematoxylin.
Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CTGF Antibody (ER1802-69)

Immunogen

Recombinant protein with human ctgf aa 150-300.

Host

Rabbit

Positive Control

Mouse smooth muscle tissue lysate, HepG2, HUVEC, SiHa, human appendix tissue, human thyroid gland cancer tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CCN family member 2

GENE NAME

CCN2

SYNONYMS

IBP-8, IGF-binding protein 8, IGFBP-8, CTGF, CCN2, HCS24, IGFBP8

SEQUENCE SIMILARITIES

Belongs to the CCN family.

TISSUE SPECIFICITY

Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix. Secreted.

FUNCTION

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.