PRODUCT CODE: ER1803-59

CTDSPL Antibody (ER1803-59)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.
  • Western blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.
  • Flow cytometric analysis of HepG2 cells with CFTR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CTDSPL Antibody (ER1803-59)

Immunogen

Recombinant protein within human ctdspl aa 50-300.

Host

Rabbit

Positive Control

HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CTD small phosphatase-like protein

GENE NAME

CTDSPL

SYNONYMS

CTDSP-like, NLI-interacting factor 1, hYA22, SCP3, Small CTD phosphatase 3, CTDSPL, C3orf8, NIF1, NIFL, SCP3, YA22

TISSUE SPECIFICITY

Expression is restricted to non-neuronal tissues.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.