PRODUCT CODE: 0807-3

COX1/Cyclooxygenase 1 Antibody (0807-3)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: 293T cell lysate, untreated<br />
Lane 2: NIH/3T3 cell lysate, untreated
  • Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: 293T cell lysate, untreated<br />
Lane 2: NIH/3T3 cell lysate, untreated
Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate, untreated
Lane 2: NIH/3T3 cell lysate, untreated

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

COX1/Cyclooxygenase 1 Antibody (0807-3)

Immunogen

Synthetic peptide corresponding to c-terminal of human cox1/cyclooxygenase 1.

Host

Rabbit

Positive Control

NIH/3T3, 293T.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500

TARGET

UNIPROT #

PROTEIN NAME

Prostaglandin G/H synthase 1

GENE NAME

PTGS1

SYNONYMS

COX-1,PGH synthase 1,PGHS-1,PHS 1

SEQUENCE SIMILARITIES

Belongs to the prostaglandin G/H synthase family.

SUBCELLULAR LOCATION

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

FUNCTION

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.