Positive control:
Lane 1: SHSY5Y cell lysate
Lane 2: HL-60 cell lysate
Applications
-
WB
-
IHC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
COPS8 Antibody (ER2001-29)
Immunogen
Recombinant protein within human cops8 aa 1-300.
Host
Rabbit
Positive Control
SHSY5Y cell lysate, HL-60 cell lysate, rat brain tissue, rat cerebellum tissue, human liver tissue, human liver carcinoma tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
Predicted band size: 23 kDa.
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:1,000
-
IHC
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
COPS8
SYNONYMS
COP9 homolog antibody; COP9 signalosome complex subunit 8 antibody; COP9 signalosome subunit 8 antibody; Cops8 antibody; CSN8 antibody; CSN8_HUMAN antibody; hCOP9 antibody; JAB1 containing signalosome subunit 8 antibody; JAB1-containing signalosome subunit 8 antibody; SGN8 antibody; Signalosome subunit 8 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Signalosome.
FUNCTION
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.