PRODUCT CODE: ER1802-88

Connexin 43 Antibody (ER1802-88)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution.
  • Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Connexin 43 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Connexin 43 Antibody (ER1802-88)

Immunogen

Synthetic peptide within c-terminal human connexin 43.

Host

Rabbit

Positive Control

Mouse testis tissue lysate, human skin tissue, human heart tissue, mouse brain tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Gap junction alpha-1 protein

GENE NAME

GJA1

SYNONYMS

Cx43, GJA1, GJAL

SEQUENCE SIMILARITIES

Belongs to the connexin family. Alpha-type (group II) subfamily.

TISSUE SPECIFICITY

Expressed in the heart and fetal cochlea.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).; Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.; S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.; Acetylated in the developing cortex; leading to delocalization from the cell membrane.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Note=Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.

FUNCTION

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).