PRODUCT CODE: ER2001-41

CLIC2 Antibody (ER2001-41)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: K562 cell lysate<br />
 Lane 2: Mouse lung tissue lysate
  • Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: K562 cell lysate<br />
 Lane 2: Mouse lung tissue lysate
  • Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Mouse lung tissue lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CLIC2 Antibody (ER2001-41)

Immunogen

Recombinant protein within human clic2 aa 80-247.

Host

Rabbit

Positive Control

K562 cell lysate, mouse lung tissue lysate, K562.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CLIC2

SYNONYMS

Chloride intracellular channel 2 antibody; Chloride intracellular channel protein 2 antibody; CLIC 2b antibody; CLIC2 antibody; CLIC2_HUMAN antibody; CLIC2b antibody; MRXS32 antibody; OTTHUMP00000196471 antibody; XAP 121 antibody; XAP121 antibody

SUBCELLULAR LOCATION

Cytoplasm, Membrane.

FUNCTION

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.Expressed in adult and fetal brain, heart, skeletal muscle, liver, lung, and spleen. Detected in adult stomach and testis. Expressed in fetal thymus and kidney.