PRODUCT CODE: ER40724

Chromogranin A Antibody (ER40724)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.
  • Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Lovo cells with chromogranin A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Chromogranin A Antibody (ER40724)

Immunogen

Peptide

Host

Rabbit

Positive Control

PC12, Lovo, mouse pancreas tissue, rat pancreas tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Chromogranin-A

GENE NAME

Chga

SYNONYMS

CgA

SEQUENCE SIMILARITIES

Belongs to the chromogranin/secretogranin protein family.

POST-TRANSLATIONAL MODIFICATION

CgA is O-glycosylated.

SUBCELLULAR LOCATION

Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.; [Serpinin]: Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Pyroglutaminated serpinin localizes to secretory vesicle.

FUNCTION

[Pancreastatin]: Strongly inhibits glucose induced insulin release from the pancreas.; [Catestatin]: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.; [Serpinin]: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. Pyroglutaminated (pGlu)-serpinin exerts an antiapoptotic effect on cells exposed to oxidative stress.

CITATIONS

  • Zhou, Yuzhen et al.

    FOXM1c promotes oesophageal cancer metastasis by transcriptionally regulating IRF1 expression. | Cell Proliferation [2019]

  • Lin, Yinuo et al.

    Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells. | Journal of Extracellular Vesicles [2020]

  • Xu, Xiaodong et al.

    Upregulation of miRNA��?01a��?p promotes tumor progression in gastric cancer by suppressing NKRF and activating NF����B signaling. | International Journal of Oncology [2020]