PRODUCT CODE: ER2001-13

CDT1 Antibody (ER2001-13)

Applications

  • WB

REACTIVITY

  • Zebrafish

Western blot analysis of CDT1 on zebrafish whole organization lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CDT1 on zebrafish whole organization lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CDT1 on zebrafish whole organization lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CDT1 Antibody (ER2001-13)

Immunogen

Synthetic peptide within zebrafish aa 200-251.

Host

Rabbit

Positive Control

Zebrafish whole organization lysates.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

76 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

CDT1

SYNONYMS

Chromatin licensing and DNA replication factor antibody; cdt1 antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

CDT1 (chromatin licensing and DNA replication factor 1) is a key licensing factor which functions to allow DNA replication. It is recruited (along with CDC6) by the ORC (Origin Recognition Complex) to sites of DNA replication and helps form the pre-replicative complex (pre-RC). Its activity during the cell cycle is regulated by its association with the Geminin protein geminin, which both inhibits CDT1 activity during S phase in order to prevent unregulated DNA replication and also to inhibit CDT1 proteolysis.