PRODUCT CODE: ER40101

CDK6 Antibody (ER40101)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2000 dilution.<br />
 Positive control: <br />
 Lane 1: K562 <br />
 Lane 2: COS-1
  • Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2000 dilution.<br />
 Positive control: <br />
 Lane 1: K562 <br />
 Lane 2: COS-1
  • ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin.
Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2000 dilution.
Positive control:
Lane 1: K562
Lane 2: COS-1

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CDK6 Antibody (ER40101)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, HepG2, COS-1, K562, human colon cancer tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

CDK6

SYNONYMS

CDK 6 antibody; CDK6 antibody; CDK6_HUMAN antibody; Cell division protein kinase 6 antibody; Crk 2 antibody; Crk2 antibody; Cyclin dependent kinase 6 antibody; Cyclin-dependent kinase 6 antibody; MGC59692 antibody; p40 antibody; PLSTIRE antibody; Serine/threonine protein kinase PLSTIRE antibody; Serine/threonine-protein kinase PLSTIRE antibody; STQTL11 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

TISSUE SPECIFICITY

Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.

POST-TRANSLATIONAL MODIFICATION

Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

CDK6 protein contains a catalytic core composed of a serine/threonine domain.This protein also contains an ATP-binding pocket, inhibitory and activating phosphorylation sites, a PSTAIRE-like cyclin-binding domain and an activating T-loop motif. CDK6 is involved in a positive feedback loop that activates transcription factors through a reaction cascade. CDK6 is important for the control of G1 to S phase transition. However, in recent years, new evidence proved that the presence of CDK6 is not essential for proliferation in every cell type, the cell cycle has a complex circuitry of regulation and the role of CDK6 might be more important in certain cell types than in others, where CDK4 or CDK2 can act as protein kinases compensating its role.