PRODUCT CODE: ET1602-17

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01] (ET1602-17)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate<br />
Lane 3: MCF-7 cell lysate
  • Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate<br />
Lane 3: MCF-7 cell lysate
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdk5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cdk5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Cdk5 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-17, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: MCF-7 cell lysate

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01] (ET1602-17)

Immunogen

Synthetic peptide within c-terminal human cdk5.

Host

Rabbit

Positive Control

Hela cell lysate, SH-SY5Y cell lysate, MCF-7 cell lysate, rat brain tissue, mouse brain tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR39-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P:1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • assay-dependent

TARGET

UNIPROT #

PROTEIN NAME

Cdk5

SYNONYMS

Cdk 5 antibody; Cdk5 antibody; CDK5_HUMAN antibody; Cell division protein kinase 5 antibody; Crk6 antibody; Cyclin dependent kinase 5 antibody; Cyclin-dependent kinase 5 antibody; Protein kinase CDK5 splicing antibody; PSSALRE antibody; Serine threonine protein kinase PSSALRE antibody; Serine/threonine-protein kinase PSSALRE antibody; Tau protein kinase II catalytic subunit antibody; TPKII catalytic subunit antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

TISSUE SPECIFICITY

Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity.; Phosphorylation at Ser-159 is essential for maximal catalytic activity.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction

FUNCTION

Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1-S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or tau protein kinase, based on its ability to phosphorylate these proteins in vitro.