PRODUCT CODE: R1309-3

CDK2 Antibody (R1309-3)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: A549<br />
 Lane 2: F9<br />
 Lane 3: PC-12<br />
 Lane 4: Mouse thymus<br />
 Lane 5: Human liver
  • Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: A549<br />
 Lane 2: F9<br />
 Lane 3: PC-12<br />
 Lane 4: Mouse thymus<br />
 Lane 5: Human liver
  • ICC staining CDK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with CDK2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: F9
Lane 3: PC-12
Lane 4: Mouse thymus
Lane 5: Human liver

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CDK2 Antibody (R1309-3)

Immunogen

Synthetic peptide within c terminal residues of human cdk2.

Host

Rabbit

Positive Control

A549, F9, PC-12, mouse thymus tissue, human liver tissue, human tonsil tissue, human breast cancer tissue, Hela.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

~33kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CDK2

SYNONYMS

Cdc2 related protein kinase antibody; cdc2-related protein kinase antibody; CDC28 antibody; CDC2A antibody; Cdk 2 antibody; CDK1 antibody; CDK2 antibody; CDK2_HUMAN antibody; CDKN2 antibody; Cell devision kinase 2 antibody; Cell division protein kinase 2 antibody; Cyclin dependent kinase 2 antibody; cyclin dependent kinase 2-alpha antibody; Cyclin-dependent kinase 2 antibody; kinase Cdc2 antibody; MPF antibody; p33 protein kinase antibody; p33(CDK2) antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.; Nitrosylated after treatment with nitric oxide (DETA-NO).

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.