PRODUCT CODE: ER80402

CD9 Antibody (ER80402)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution.
  • Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution.
  • ICC staining CD9 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD9 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD9 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-CD9 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with CD9 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD9 Antibody (ER80402)

Immunogen

Peptide

Host

Rabbit

Positive Control

A549, Hela, Jurkat, HepG2, Lovo, human lung cancer tissue, human colon cancer tissue, mouse large intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD9

SYNONYMS

Tetraspanin 29 antibody; 5H9 antibody; 5H9 antigen antibody; Antigen defined by monoclonal antibody; 602 29 antibody; Antigen defined by monoclonal antibody; 60229 antibody; BA-2/p24 antigen antibody; BA2 antibody; BTCC 1 antibody; BTCC1 antibody; CD9 antibody; CD9 antigen antibody; CD9 antigen p24 antibody; CD9 molecule antibody; CD9_HUMAN antibody; Cell growth inhibiting gene 2 protein antibody; Cell growth-inhibiting gene 2 protein antibody; DRAP 27 antibody; DRAP27 antibody; GIG2 antibody; Growth inhibiting gene 2 protein antibody; Leukocyte antigen MIC3 antibody; MIC3 antibody; Motility related protein antibody; Motility-related protein antibody; MRP 1 antibody; MRP-1 antibody; MRP1 antibody; p24 antibody; p24 antigen antibody; Tetraspanin-29 antibody; Tspan 29 antibody; Tspan-29 antibody; TSPAN29 antibody

SEQUENCE SIMILARITIES

Belongs to the tetraspanin (TM4SF) family.

TISSUE SPECIFICITY

Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Palmitoylation by ZDHHC2 regulates CD9 expression, association with other tetraspanin family proteins and function in cell adhesion.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. Protein exists in three forms, and is known to carry covalently linked fatty acids. It is involved in platelet activation, aggregation, in cell adhesion, cell motility and tumor metastasis. It regulates paranodal junction formation, and is also required for gamete fusion.